Nikon a1 confocal manual

Confocal Nikon a1R Spectral Nikon A1r spectral Imaging live (short time-lapses <2 hours) and ˜xed samples - any ˜uorescent proteins Depth (z) Speed acq. Resolution Bleaching Multicolor high good medium 4-8 <30 um Temperature, Humidity, CO2 control Peristaltic pum, water bath 3D Z-stacks, tiled images Colocalization, F-techniques (FRAP, FRET,) RCF Nikon Confocal & SIM User Manual. Publications using N-SIM Super Resolution Microscope System . Nikon Confocal and superresolution microscopy e-book (Science/AAAS Publishing group) Nikon Microscopy Education Objectives. Obj. Objective. Medium. Use. 10x. Plan Apo 10x/0.45NA. Dry. Confocal. 20x. Nikon A1R FLIM Confocal Microscope Location: 1-220H CCRB Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. CONFOCAL PLATFORM (Nikon A1R-HD, 2018): 4 diode Lasers (405 nm, 488nm, 561 nm, 640 nm) 4 PMTS (Standard: Blue and Far Red, GaASP: Green and Red) 32 channel spectral detector. Transmitted light Detector for Differential Interference Contrast (DIC) microscopy. NIS Elements Advanced software v 5.2 This was the beginning of a revolutionary line of confocal microscopes that would span years and help bring Confocal microscopy to labs all over the world. This system, combined with Nikon's flagship TE2000 inverted microscope, boasted an ultra-compact, state of the art multi-channel laser confocal with an easy to use software interface all Nikon Microscope Documentation. Brochures; Eclipse E200 Brochure; Eclipse Epi-Fluorescence Brochure; Reflected Light Components; Episcopic Objectives; C2+ Confocal; Operation Manuals; Finite Conjugate; Model S-Kt; Diaphot TMD; Epiphot TME; Labophot Biological; Optiphot Biological; A1 Confocal; Accessories; Eclipse TE2000 DIC; Eclipse The Nikon A1si is a confocal system equipped with a point-scan scan head, 5 standard PMT detectors and a 32-channel PMT spectral detector. The A1si's highest resolution mode is up to 4096 x 4096 1. First, set your confocal settings as needed for imaging. 2. Open the "A1 Stimulation" and "ND Stimulation" dialog boxes under View Acquistion Controls on the top toolbar. 3. Set up as shown below. Select your bleaching laser (405) and adjust power (usually 100%) 4. The ND experiment should be saved to file (choose your location). Inverted wide field fluorescence scope with color camera and DIC optics. Manual stage control. This workhorse tissue culture microscope features advanced optical design for the shortest, most efficient light path. Incorporated as a part of the built-in turret assembly is a focusable Bertrand lens, photomask reticle and dark slide. NikonÕs modular A1 + /A1R + confocal laser scanning microscope system can meet the most demanding imaging requirements with hardware and capabilities that are continuously updated to achieve both high-quality images for spatial information and high speed images of fast-moving events. Smart Tools for Superior Results Optimized sensitivity The Nikon A1R is a point scanning confocal microscope based around a fully motorized, inverted Nikon Ti body with hardware-enabled focus correction. The Nikon A1R has three photomultipliers for fluorescence imaging, one for transmitted light detection and a dedicated photomultiplier array for spectral profil