Pcr2 1 topo manual tire

The controls show the amplified PCR product (lane 3) and vector without insert (lane 4). M: marker λ-EcoRI +HindIII-Marker-Mix 3 (Fermentas, St. Leon-Rot) 1 µg. The inserts are ligated into pCR 2.1-TOPO and contain BsaI sites, The bands at ~1kb are pieces of the vector (pCR2.1-TOPO), which contains a BsaI site Sequence analysis of the transposon insertion regions revealed the pCR2.1-TOPO (Invitrogen) and transformed into Escherichia coli TOP10 (In- Gel